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Gellan gum is a water-soluble anionic polysaccharide produced by the bacterium Sphingomonas elodea (formerly Pseudomonas elodea). The gellan-producing bacterium was discovered and isolated by the former Kelco Division of Merck & Company, Inc. in 1978 from the lily plant tissue from a natural pond in Pennsylvania, USA. It was initially identified as a substitute gelling agent at significantly lower use level to replace agar in solid culture media for the growth of various microorganisms Its initial commercial product with the trademark as "GELRITE" gellan gum, was subsequently identified as a suitable agar substitute as gelling agent in various clinical bacteriological media.

Gelzan™ is produced by fermentation and an agar substitute also known as Gellan Gum. Use at 2-4 g/L. Do not use with media containing high levels of calcium such as DKW and Quoirin & Lepoivre. Also if divalent cation (Ca²⁺, Mg²⁺) concentrations are below that of 1/2X MS (e.g., our Orchid Maintenance Medium with Charcoal and without Agar) you may need to use 10-12 g/L to obtain free-standing gels. This gelling agent requires melting at 121°C. If your autoclave doesn't reach that temperature for 15-20 min the final gel may appear clumpy.